dMRI: Preprocessing


This script,, demonstrates how to prepare dMRI data for tractography and connectivity analysis with nipype.

We perform this analysis using the FSL course data, which can be acquired from here:

Can be executed in command line using python

Import necessary modules from nipype.

import os  # system functions
import as nio  # Data i/o
import nipype.interfaces.utility as niu  # utility
import nipype.algorithms.misc as misc

import nipype.pipeline.engine as pe  # pypeline engine

from nipype.interfaces import fsl
from nipype.interfaces import ants

Load specific nipype’s workflows for preprocessing of dMRI data: niflow.nipype1.workflows.dmri.preprocess.epi.all_peb_pipeline, as data include a b0 volume with reverse encoding direction (P>>>A, or y), in contrast with the general acquisition encoding that is A>>>P or -y (in RAS systems).

from niflow.nipype1.workflows.dmri.fsl.artifacts import all_fsl_pipeline, remove_bias

Map field names into individual subject runs

info = dict(
    dwi=[['subject_id', 'dwidata']],
    bvecs=[['subject_id', 'bvecs']],
    bvals=[['subject_id', 'bvals']],
    dwi_rev=[['subject_id', 'nodif_PA']])

infosource = pe.Node(
    interface=niu.IdentityInterface(fields=['subject_id']), name="infosource")

# Set the subject 1 identifier in subject_list,
# we choose the preproc dataset as it contains uncorrected files.
subject_list = ['subj1_preproc']

Here we set up iteration over all the subjects. The following line is a particular example of the flexibility of the system. The datasource attribute iterables tells the pipeline engine that it should repeat the analysis on each of the items in the subject_list. In the current example, the entire first level preprocessing and estimation will be repeated for each subject _list.

infosource.iterables = ('subject_id', subject_list)

Now we create a object and fill in the information from above about the layout of our data. The Node module wraps the interface object and provides additional housekeeping and pipeline specific functionality.

datasource = pe.Node(
    nio.DataGrabber(infields=['subject_id'], outfields=list(info.keys())),

datasource.inputs.template = "%s/%s"

# This needs to point to the fdt folder you can find after extracting
datasource.inputs.base_directory = os.path.abspath('fdt1')
datasource.inputs.field_template = dict(
    dwi='%s/%s.nii.gz', dwi_rev='%s/%s.nii.gz')
datasource.inputs.template_args = info
datasource.inputs.sort_filelist = True

An inputnode is used to pass the data obtained by the data grabber to the

inputnode = pe.Node(
    niu.IdentityInterface(fields=["dwi", "bvecs", "bvals", "dwi_rev"]),

Setup for dMRI preprocessing

In this section we initialize the appropriate workflow for preprocessing of diffusion images.

Artifacts correction

We will use the combination of topup and eddy as suggested by FSL.

In order to configure the susceptibility distortion correction (SDC), we first write the specific parameters of our echo-planar imaging (EPI) images.

Particularly, we look into the acqparams.txt file of the selected subject to gather the encoding direction, acceleration factor (in parallel sequences it is > 1), and readout time or echospacing.

epi_AP = {'echospacing': 66.5e-3, 'enc_dir': 'y-'}
epi_PA = {'echospacing': 66.5e-3, 'enc_dir': 'y'}
prep = all_fsl_pipeline(epi_params=epi_AP, altepi_params=epi_PA)

Bias field correction

Finally, we set up a node to correct for a single multiplicative bias field from computed on the b0 image, as suggested in [Jeurissen2014].

bias = remove_bias()

Connect nodes in workflow

We create a higher level workflow to connect the nodes. Please excuse the author for writing the arguments of the connect function in a not-standard style with readability aims.

wf = pe.Workflow(name="dMRI_Preprocessing")
wf.base_dir = os.path.abspath('preprocessing_dmri_tutorial')
wf.connect([(infosource, datasource, [('subject_id', 'subject_id')]),
            (datasource, prep,
             [('dwi', 'inputnode.in_file'), ('dwi_rev', 'inputnode.alt_file'),
              ('bvals', 'inputnode.in_bval'), ('bvecs', 'inputnode.in_bvec')]),
            (prep, bias, [('outputnode.out_file', 'inputnode.in_file'),
                          ('outputnode.out_mask', 'inputnode.in_mask')]),
            (datasource, bias, [('bvals', 'inputnode.in_bval')])])

Run the workflow as command line executable

if __name__ == '__main__':



Jeurissen et al., Multi-tissue constrained spherical deconvolution for improved analysis of multi-shell diffusion MRI data. NeuroImage 103:411–426. 2014. doi:10.1016/j.neuroimage.2014.07.061.

Example source code

You can download the full source code of this example. This same script is also included in Nipype1 Examples Niflow under the package/niflow/nipype1/examples directory.